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Description: Mycoplasma is a common and serious contaminant of cell cultures. It has been shown that more than 70% of cell cultures in the laboratory are infected with mycoplasma. In continuous cell cultures, contaminating mycoplasma may grow slowly without killing the cells but affecting various parameters including altered cellular proliferation and viability, morphological changes, cell transformation, mimicking virus infection, and inresponsiveness to drug treatment, etc., and ultimately leading to unreliable results. Naturally, the ideal solution is to discard the contaminated cultures. However, if the cells that are stored in liquid nitrogen are also contaminated, the only option is to eliminate the mycoplasma, particularly if the cells are unique, which require extensive work to re-develop. A number of effective methods for the elimination of mycoplasma contamination in cell cultures have been developed, the preferred method in terms of simplicity is treatment with antibiotics, which minimize the damages from other treatments. However, common antibiotics used in cell cultures such as penicillin, which attacks bacterial cell walls, are ineffective in this instance, since mycoplasma lacks a cell wall. Several antibiotics including Neomycin, Tetracycline and Gentamicin have been proven to be effective to eliminate mycoplasma. However, the efficacy of these antibiotics is restricted to specific mycoplasma species and often suppress mycoplasma proliferation rather than disinfect the cells. For this reason, once treatment is concluded, contamination will recur.
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