EnvSeptic (spray)
保存条件
Description:
Mycoplasma is a common and serious contaminant of cell
cultures. It has been shown that more than 70% of cell
cultures in the laboratory are infected with mycoplasma. In
continuous cell cultures, contaminating mycoplasma may
grow slowly without killing the cells but affecting various
parameters including altered cellular proliferation and
viability, morphological changes, cell transformation,
mimicking virus infection, and inresponsiveness to drug
treatment, etc., and ultimately leading to unreliable results.
Naturally, the ideal solution is to discard the contaminated
cultures. However, if the cells that are stored in liquid
nitrogen are also contaminated, the only option is to
eliminate the mycoplasma, particularly if the cells are unique,
which require extensive work to re-develop.
A number of effective methods for the elimination of
mycoplasma contamination in cell cultures have been
developed, the preferred method in terms of simplicity is
treatment with antibiotics, which minimize the damages from
other treatments. However, common antibiotics used in cell
cultures such as penicillin, which attacks bacterial cell walls,
are ineffective in this instance, since mycoplasma lacks a cell
wall. Several antibiotics including Neomycin, Tetracycline and
Gentamicin have been proven to be effective to eliminate
mycoplasma. However, the efficacy of these antibiotics is
restricted to specific mycoplasma species and often suppress
mycoplasma proliferation rather than disinfect the cells. For
this reason, once treatment is concluded, contamination will
recur.
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