Krebs-Ringer Bicarbonate Buffer has a variety of laboratory uses including tissue cell culture, washing, primary cell preparation and bioassays. The salts in this buffer provide water and inorganic ions to the cells, which in turn maintains osmotic balance and the desired pH in the culture medium. All media used in tissue culture are based on synthetic mixtures of inorganic salts, which are known as physiological or balanced salt solutions (BSS).